Remifentanil promotes osteoblastogenesis by upregulating Runx2/osterix expression in preosteoblastic C2C12 cells
윤지영, 김태성, 안지혜, 윤지욱, 김형준, 김은정,
소속 상세정보
윤지영 ( Yoon Ji-Young ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
김태성 ( Kim Tae-Sung ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
안지혜 ( Ahn Ji-Hye ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
윤지욱 ( Yoon Ji-Uk ) - Pusan National University School of Medicine Department of Anesthesia and Pain Medicine
김형준 ( Kim Hyung-Joon ) - Pusan National University School of Dentistry Department of Oral Physiology
김은정 ( Kim Eun-Jung ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
Abstract
Background: The imbalance between osteoblasts and osteoclasts can lead to pathological conditions such as osteoporosis. It has been reported that opioid adversely affect the skeletal system, but it is inconsistent. Remifentanil is currently used as an adjuvant analgesic drug in general anesthesia and sedation. The aim of the present study was to investigate the effect of remifentanil on the osteoblast differentiation and mechanism involved in this effect.
Methods: The C2C12 cells (mouse pluripotent mesenchymal cell line) were used as preosteoblast. Osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) staining. C2C12 cell migration by remifentanil was evaluated using Boyden chamber migration assay. The expression of Runx2 and osterix was evaluated by RT-PCT and western blot analysis to investigate the mechanism involved in remifentanil-mediated osteoblast differentiation.
Results: ALP staining showed that remifentanil increased significantly osteoblast differentiation. In Boyden chamber migration assay, C2C12 cell migration was increased by remifentanil. RT-PCR and western blot analysis showed that the expression of Runx2 and osterix was upregulated by remifentanil.
Conclusions: We demonstrated that remifentanil increased osteoblast differentiation in vitro by upregulation of Runx2 and osterix expression. Therefore, remifentanil has the potential for assisting with bone formation and bone healing.
키워드
Osteoblasts; Osterix; Remifentanil; Runx2
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